Alexa Fluor 488® Secondary Antibodies

2024-03-16 23:21| 来源: 网络整理| 查看: 265

Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information).

Alexa Fluor® 488 is a versatile dye and can be combined with different dyes for effective 4 color immunofluorescence.

4 color staining with DyLight™ 405, Rhodamine Red™-X and Alexa Fluor® 647 4 color staining with Alexa Fluor® 555 or 568 Alpha tubulin visualised with 115-545-205 (Alexa Fluor® 488 Goat Anti-Mouse IgG, Fcγ subclass 1 specific). Image by David Robertson, ICR.

Alexa Fluor® 488 is available conjugated to:

Whole IgG Secondary Antibodies F(ab')2 Fragment Secondary Antibodies Fab Fragment Secondary Antibodies FabuLight™ - Fc Specific Fab Fragments for Labeling Primary Antibodies In Solution Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP Antibodies Streptavidin ChromPure™ Purified Proteins from Normal Serums Dye Laser line (nm) Common filter set Excitation (nm) Emission (nm) Extinction co-efficient Quantum Yield (Φ) Molecular Weight (kDa) Applications Alexa Fluor® 488 488 530/30 493 519 1.4 0.92 643 Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting Dye Alexa Fluor® 488 Laser line (nm) 488 Common filter set 530/30 Excitation (nm) 493 Emission (nm) 519 Extinction co-efficient 1.4 Quantum Yield (Φ) 0.92 Molecular Weight (kDa) 643 Applications Microscopy SRM Flow Cytometry Fluorisa Fluorescent Western Blotting Suggested Dilution Factors Alexa Fluor® 488 Conjugated Antibodies Control Reagents Product Code Alexa Fluor® 488-conjugated ChromPure Bovine IgG, whole molecule 001-540-003 Alexa Fluor® 488-conjugated ChromPure Chicken IgY (IgG), whole molecule 003-540-003 Alexa Fluor® 488-conjugated ChromPure Donkey IgG, F(ab')2 fragment 017-540-006 Alexa Fluor® 488-conjugated ChromPure Donkey IgG, whole molecule 017-540-003 Alexa Fluor® 488-conjugated ChromPure Goat IgG, F(ab')2 fragment 005-540-006 Alexa Fluor® 488-conjugated ChromPure Goat IgG, whole molecule 005-540-003 Alexa Fluor® 488-conjugated ChromPure Human Albumin 009-540-051 Alexa Fluor® 488-conjugated ChromPure Human IgG, F(ab')2 fragment 009-540-006 Alexa Fluor® 488-conjugated ChromPure Human IgG, Fab fragment 009-540-007 Alexa Fluor® 488-conjugated ChromPure Human IgG, Fc fragment 009-540-008 Alexa Fluor® 488-conjugated ChromPure Human IgG, whole molecule 009-540-003 Alexa Fluor® 488-conjugated ChromPure Human IgM (myeloma), whole molecule 009-540-012 Alexa Fluor® 488-conjugated ChromPure Human Transferrin 009-540-050 Alexa Fluor® 488-conjugated ChromPure Mouse IgG, F(ab')2 fragment 015-540-006 Alexa Fluor® 488-conjugated ChromPure Mouse IgG, whole molecule 015-540-003 Alexa Fluor® 488-conjugated ChromPure Mouse Transferrin 015-540-050 Alexa Fluor® 488-conjugated ChromPure Rabbit IgG, whole molecule 011-540-003 Alexa Fluor® 488-conjugated ChromPure Rat IgG, whole molecule 012-540-003 Alexa Fluor® 488-conjugated ChromPure Rat Transferrin 012-540-050 Alexa Fluor® 488-conjugated ChromPure Sheep IgG, whole molecule 013-540-003 Streptavidin Product Code Alexa Fluor® 488-conjugated Streptavidin 016-540-084 Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

Read Our Blog About Selecting Fluorophores Using the Spectra Viewer Show Excitation and Emission Spectra Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp.

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